3D visualisation of voids in grapevine flowers and berries using X‐ray micro computed tomography

Background and Aim X-ray micro computed tomography (micro-CT) is a non-destructive 3D imaging technique that has been applied to plant morphology anatomical studies gain better understanding of physiological phenomena in vivo. It particularly useful for voids undisturbed fragile tissues therefore may be the delicate flowers soft berries Vitis vinifera. The characterisation gas spaces channels can offer insights into process tissue aeration this have implications on cell function vitality. We assessed use micro-CT visualise within these reproductive organs. Methods Results internal structures were captured through rapid scanning subsequently recreated using image processing. relative positions developing flower parts encased cap visualised. Low density/porous was identified pedicel receptacle, connecting lenticels with interior berry. Voids present proximal mesocarp mature forming ‘detachment zone’ both seeded seedless cultivars. permeated grape cultivars, but not grapes. Conclusion Micro-CT offers new regarding distribution compositional heterogeneity organs are difficult dissect and/or view light microscopy. Significance Study A physiology functionality grapevine achieved by visualisation structure